New Efficient Reagent For Stem Cell Transfection

Online, February 21, 2013 (Newswire.com) - OZ Biosciences SAS, specialized in the development of innovative transfection technologies is delighted to announce the launching of his new product DreamFect™ Stem.

Stem cells are widely investigated due to their regenerative potential. In many cases, it would prove useful to enhance or silence some of their features through gene delivery strategies to harness their full potential cell/gene-based therapy. Because stem cells behavior, physiology and properties are quite distinctive to other cells, OZ Biosciences has developed a specific transfection reagent for stem cells.
DreamFect™ Stem exhibits high transfection efficiency on human adult multipotent stem cells such as MSC (Mesenchymal Stem Cells), AdSC (Adipose derived Stem Cells), AFSC (Amniotic Fluid Stem Cells), and KG1a.

DreamFect™ Stem has main advantages in comparison to standard procedures:

1. High transfection efficiency for multipotent Stem cells.

2. Minimized toxicity due to reagent biodegradability and low DNA amount required.

3. Cell phenotype and differentiation potential are not affected.
Human mesenchymal stem cells transfected with DreamFectTM Stem retain their potential to differentiate into osteoblasts when cultured in osteogenic differentiation medium.

4. Serum Compatible.
It has been shown that working in serum-free medium may lead to spontaneous cell differentiation of various stem cells and to heterogeneity within the treated cell population. DreamFect™ Stem allows you to work in complete medium and to avoid this kind of disagreement.

5. Simple, Ready-to-use and rapid (no specific buffer):

- Mix 3μL of DreamFectTM Stem Transfection reagent per μg of DNA (no specific dilution buffer needed)

- Incubate 20 min at room temperature

- Add the complexes to your cell growing in complete medium

DreamFect™ Stem transfection reagent is provided with a comprehensive protocol that contains all information for your Stem Cell transfection.